52. Effects of CBMIDA on Removal of Uranium in Rats
Satoshi Fukuda, Haruzo Iida,
Xueming Yan* and Yuyuan Xie*
(*Shanghai Institute of Materia Medica, China)
Keywords: CBMIDA, uranium, rat
The effects of a chelating agent, CBMIDA, on the removal of uranium in rats were determined. Thirty rats were divided into six groups. Group 1(G1): rats injected intraperitonially with uranium in a solution of pH 3.2 and injected with sodium bicarbonate 30 min later; Group 2 (G2): uranium compounded with CBMIDA before injection; Group 3 (G3): uranium in a pH 3.2 solution; Group 4 (G4): uranium in a pH 3.2 solution and injection of 1200 µmol/kg CBMIDA 30 min later, Group 5: uranium in a pH 6.8 solution; and Group 6: uranium in a pH 6.8 and injection of 1200 µmol/kg CBMIDA. The uranium activities in the excreta and organs in rats 24 hrs after injection were measured.
The rates of uranium excreted were 1.05 % of the injected dose in group G1, 1.00% in G3, and 0.24% in G5, whereas they were 11.26% in group G2, 9.49% in G4, and 10.13% in G6, respectively (Table 1). The amount of activity in excreta (urine and feces) was highest in group G2. A significant difference of activity in the excreta between groups G 5 and G6 was found. The retention rates in the liver, femur, and spleen in groups G2, G4, and G6, except for the value of spleen in G4, decreased more than those of the corresponding G1, G3 and G5 groups, respectively. However, the retention rate in the kidney in groups G2 and G4 was higher than that in groups G1 and G3 respectively, but the rate in the G6 group was lower than that of G5.
In conclusion, the results indicate that CBMIDA can remove uranium at pH6.8 from the body, while its has no effect for uranium at pH3.2 solution and shows undesirable characteristic of accumulating uranium in the kidney. Further study of the analogues of CBMIDA will be necessary in order to enhance its removal effects and reduce the associated accumulation of radioactive materials from the kidney.
Publications:
Fukuda, S., Iida, H., Yan, X. and Xie, Y.: Biomarkers and Environment, 4, Suppl.1 35-37, 2001.
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