40. Comparison Study on Genome Coding DNA-dependent Protein Kinase between Chickens and Mice

Masumi Abe, Akira Fujimori, Ryutaro Fukumura, Tatsuya Ohhata, Ryoko Araki, Toshiyuki Saito, Hirokazu Takahashi and Yoko Tsutsumi

Keywords: chickens, mice, DNA-PKcs gene

The gene of catalytic subunit of DNA dependent protein kinase is responsible gene for SCID mice. The molecules play a critical role in non-homologous end joining including the V(D)J recombination. Contribution of the molecules to the difference of radiosensitivity and the susceptibility to cancer has been suggested. Here we show the entire nucleotide sequence of approximately 193 kbp and 84 kbp genomic regions encoding the entire DNA-PKcs gene in the mouse and chicken, respectively. Retroposon was found in intron 51 of mouse genomic DNA-PKcs gene but not in human and chicken. Comparative analysis of these two species strongly suggested that only two genes, DNA-PKcs and MCM4, exist in the region of both species. Several conserved sequences and cis elements, however, were predicted.

Publications:
1) Fujimori, A., Hashimoto, H., Araki, R., Saito, T., Kasama, Y., Mori, M., Fukumura, R., Ohhata, T., Tatsumi, K. and Abe, M. Rad. Res. 157, 298-305, 2002.
2) Ohhata T., Araki R., Fukumura R., Kuroiwa A., Matsuda Y. and Abe M. Gene. 280, 59-66, 2001.
3) Fukushima T., Takata M., Morrison C., Araki R., Fujimori A., Abe M., Tatsumi K., Jasin M., Dhar P.K., Sonoda E., Chiba T. and Takeda S. J. Biol. Chem. 276, 44413-44418, 2001.