Annual Report

16. Effects of Antibodies of Growth Factors and Cytokines on the Proliferation of Cultured Epidermal Melanoblasts and Melanocytes Derived from Skins of UVB-Induced Pigmented Spots in Mice

Tomohisa Hirobe

Keywords: melanoblast, melanocyte, antibody, UVB, proliferation


Long-term exposure to ultraviolet radiation B (UVB) induced pigmented spots in the dorsal skin of hairless mice. It has been shown that the proliferation of epidermal melanoblasts and melanocytes derived from skins of UVB-induced pigmented spots is greatly stimulated by using a serum-free culture system supplemented with dibutyryl adenosine 3':5'-cyclic monophosphate (DBcAMP) and basic fibroblast growth factor (bFGF). In this study, to understand what factors are involved in regulating the proliferation of cultured epidermal melanoblasts and melanocytes, numerous antibodies against growth factors and cytokines were added in the serum-free medium from the initiation of primary cuture. Results showed that antibodies against endothelin-1, -2, -3, granulocyte macrophage colony stimulating factor (GMCSF), stem cell factor (SCF) inhibited the proliferation of cultured mouse epidermal melanoblasts and melanocytes derived from skins of UVB-induced pigmented spots. These results suggest that numerous factors are involved in regulating the proliferation of melanoblasts and melanocytes from skins of UVB-induced pigmented spots.

Publications:
T. Hirobe and H. Abe: J. Exp. Zool., 286, 632-640, 2000.
T. Kunisada, H. Yamazaki, T. Hirobe, S. Kamei, M. Omoteno, H. Tagaya, H. Hemmi, U. Koshimizu, T. Nakamura, and S. Hayashi: Mech. Dev. 94, 67-78, 2000.


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