33. Evidence for mRNA Expression of Vascular Endothelial Growth Factor by X-ray Irradiation in a Lung Squamous Carcinoma Cell Line

Koichi.Ando, Soichiro Ando, Kumie Nojima, Hideyuki Majima, Hiroshi Ishihara, Masao Suzuki, Yoshiya Furusawa, Sachiko Koike, Hiroshi Yamaguchi and Masatake Yamauchi

Keywords: VEGF, cytokine, inhibitor, Src tyrosine kinase, protein kinase C

Vascular endothelial growth factor (VEGF) is a multipotent cytokine which plays an important role in various angiogenic conditions as well as in some tumor behaviors. Here we examined the induction of VEGF mRNA by X-ray irradiation in a lung squamous cell carcinoma cell line (RERF-LC-AI). Irradiating the cells with 15 Gy X-rays significantly increased the mRNA expression up to 2.5-fold more than the control at a post-irradiation time of 16-24 h. The induction of VEGF mRNA by X-rays irradiation was completely blocked by treating cells with either genestein (Src tyrosine kinase inhibitor) or H7 (protein kinase C inhibitor). This suggests that the mechanism of induction might be concerned with the pathway which triggers Src tyrosine kinase of the cell surface and the protein kinase C pathway.

Publications:
Ando S., Nojima K., Majima H., Ishihara H., Suzuki M., Furusawa Y., Yamaguchi H., Koike S., Ando K., Yamauchi M. and Kuriyama T. : Cancer Letters 132, 75-80, 1998